![]() The GC content and CAI value of the vaccine construct were calculated as 55 and 0.97, respectively. coli (strain K12) were conducted by JCat. Back translation and optimization of the codon usage of vaccine sequence in E. You can also leave us a feedback at any time or send your requests for further enhancements or features. Codon optimization and in silico cloning. Online support - accessible easily within the software.Using Genome Compiler is simple and intuitive, but these tools will help you become a Genome Compiler expert faster: Every change made to the data you share is trackable and can be easily accessed. You can share your primers, sequences and projects with others and let them edit and add their comments. Using Genome Compiler, you can work efficiently with your friends and colleagues at the lab. Fixed an issue where after resurrecting some very old sequences you could be forced to save when closing if SnapGene was configured to update older files automatically. It is also possible to query the NCBI database from within Genome Compiler and then instantly import your data into the Materials Box directly from Genome Compiler. In addition, there is an auto annotation library, containing the Plasmapper database of parts, including hundreds of commonly used coding sequences, promoters and terminators and more, which can be “Blasted” against to auto annotate your sequences. Inside Genome Compiler Materials Box you'll find thousands of sequences, plasmids and parts from different repositories: Gene Synthesis & Cloning/Mutagenesis FAQs The Most Commonly Asked Questions Ordering Service Details Recombinant Antibody Production Codon Optimization. Back translation and codon optimization.Virtual digest and gel electrophoresis results simulation.RBS Calculator - predict and control the protein expression rate in bacteria by this tool by the Salis lab, which is embedded into Genome Compiler.Using the software you can also create a Primer Library in order manage your inventory and automatically attach complementary primers to your projects. Primer design - design primers automatically or manually.You can use our extensive library or add your own. Sequence annotation - seamlessly auto-annotate your sequences and FASTA files.scf) against your template sequence, locate and correct errors instantly. Sequence alignment - simultaneously align your chromatogram files (.ab1.The software supports Restriction Ligation (for digest and ligate using restriction enzymes or PCR) and Gibson Assembly methods. Cloning - design your cloning experiments by the intuitive cloning wizards.
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